运动后尿液蛋白质分子量与等电点的变化特征

通过对９名男性受试者在分别完成１００－２００ｍ，４００－８００ｍ和１ ５００－３ ０００ｍ跑步间歇训练后尿蛋白分子量和等电点的测定发现：①运动时尿液高、低分子量蛋白质排泄率均较运动前明显增加，但以高分子量蛋白质排泄为主；②运动时尿液高、低分子量蛋白质排泄率均以４００－８００ｍ间歇训练时最高，１００－２００ｍ间歇训练时次之，１ ５００－３ ０００ｍ间歇训练时最低；③运动时尿液排出的蛋白质以负离子为主

THE CHARACTERISTICS OF CHANGES OF URIANARY PROTEIN MOLECULAR WEIGHT AND ISOELECTRIC POINT IN POST-EXERCISE URINE

Nine young male volunteers participated IN three run-training of differnt intensities(100 200m, 400 800m and 1 500 3000m)at 90% of their maximum performance. The pre-andpost-training urine samples were collected to determine the protein molecular weight andisoelectric point.Quantitative analysis showed that(1) both high and low molecular weight pro-tein excretion increased significantly, with the high molecular weight proteins being the dominantcomponent;(2) excretion rates of both high and low molecular weight urinary proteins increasedsignificantly after running as compared with pre-training values, with the values after 400-800mtraining being the highest and those after 1500-3000m training being the lowest;(3)most of ex-creted urinary proteins were negative charged and the IEP patterns were very similar among thethree different training.The results suggest that increased glomerular fiItration membranepermeability and impaired tubular protein reabsorption may be the two direct causes ofexercise-induced proteinuria and the former factor may has certain relation to the impairedglomerular electrostatic barrier.