Changes in M:G ratios of extracted and residual alginate fractions on boiling with water the dried brown alga Kjellmaniella crassifolia (Laminariales,Phaeophyta) |
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Authors: | Nishide Eiichi Anzai Hirosi Uchida Naozuki Nisizawa Kazutosi |
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Institution: | (1) College of Agriculture and Veterinary Medicine, Nihon University, Shimouma, 154 Setagaya, Tokyo, Japan |
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Abstract: | Kjellmaniella crassifolia, the edible macro-brown alga in Japan contained nearly 27% of alginates of which nearly 7% was extractable from the fronds with boiling water for 6 h and the residual alginates in the frond were almost exhaustively extracted with a dilute alkali at 60 °C for 6 h. The alginates dissolved in all these extracts with both boiling water and dilute alkali were purified by fractionation with MgCl2 and alcohol.The content of MM blocks in the boiling water-soluble alginate sample increased remarkably during heating for 6 h while that of GG blocks from the same sample decreased. In contrast, MM blocks in the alkali-soluble alginate sample decreased during 6 h heating while GG blocks continued to increase. Since the amounts of MG blocks showed slight fluctation, the M:G ratio of alginates extracted with boiling water increased towards the end of extraction whereas the reverse is true for the alkali-soluble alginates. |
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Keywords: | alkali-soluble alginate block structure boiling water-soluble alginate brown alga Kjellmaniella crassifolia M:G ratio seaweed |
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