Isolation and characterization of a bacterium possessing a novel aldoxime-dehydration activity and nitrile-degrading enzymes |
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Authors: | Yasuo Kato Ryoko Ooi Y Asano |
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Institution: | (1) Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Toyama 939–03, Japan e-mail: asano@pu-toyama.ac.jp Tel. +81-766-56-7500, ext. 530; Fax +81-766-56-2498, JP |
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Abstract: | A bacterial strain capable of utilizing E-pyridine-3-aldoxime as a nitrogen source was isolated from soil after a 4-month acclimation period and was identified as
Rhodococcus sp. The strain contained a novel aldoxime dehydration activity that catalyzed a stoichiometric dehydration of E-pyridine-3-aldoxime to form 3-cyanopyridine. The enzyme activity was induced by various aldoximes and nitriles. The strain
metabolized the aldoxime as follows: E-pyridine-3-aldoxime was dehydrated to form 3-cyanopyridine, which was converted to nicotinamide by a nitrile hydratase,
and the nicotinamide was successively hydrolyzed to nicotinic acid by an amidase.
Received: 21 January 1998 / Accepted: 12 May 1998 |
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Keywords: | Aldoxime dehydration Synthesis Aldoxime Nitrile Rhodococcus sp |
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