Terminal respiration in certain mammalian trypanosomes |
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| Authors: | FULTON J D SPOONER D F |
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| Affiliation: | 1. Departamento de Biología, Facultad de Ciencias, Universidad de Los Andes, Mérida 05101, Estado Mérida, Venezuela;2. Departamento de Biología, Facultad Experimental de Ciencias, Universidad del Zulia, Maracaibo, Estado Zulia, Venezuela;1. Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil;2. Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, SP, Brazil;3. Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brazil;3. Department of Microbiology and Molecular Genetics, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, Texas 77030;4. Max F. Perutz Laboratories, Vienna Bio-center, Medical University of Vienna, Dr. Bohr-Gasse 9, 1030 Vienna, Austria;1. Foundation for Innovative New Diagnostics, Geneva 1202, Switzerland;1. Foundation Medicine, 7010 Kit Creek Road, Morrisville, NC 27560, USA;2. Division of Hematology/Oncology, The University of North Carolina at Chapel Hill, CB #7305, Chapel Hill, NC 27599-7305, USA;3. Department of Pathology and Laboratory Medicine, The University of North Carolina at Chapel Hill, CB #7525, Chapel Hill, NC 27599-7525, USA |
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| Abstract: | - 1.1. Specific methods have been used with 17 strains of mammalian trypanosomes to find if the presence or absence of a cytochrome system is correlated with the biological classification of these organisms.
- 2.2. A cytochrome system was observed in insect and blood forms of trypanosomes of the lewisi group.
- 3.3. No evidence of this system was found in the vivax, congolense or brucei groups when care was taken to remove contaminating host-blood cells.
- 4.4. In contrast such a system was present in the insect form of T. gambiense, which was the only species examined.
- 5.5. Manometric and spectrophotometric assays of cytochrome oxidase in a number of trypanosomes in which this system was present have shown surprisingly low values, in view of Qo2 values of these organisms.
- 6.6. Homogenates of blood stream forms of T. rhodesiense oxidized DPNH by a system in which cytochrome was absent and formation of peroxide could not be detected.
- 7.7. The respiration of all the strains examined was markedly sensitive to either added peroxide or that formed in situ. This sensitivity was therefore not correlated with the action of thiol group inhibitors.
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