Growth of mixed cultures of Actinomyces viscosus and Streptococcus mutans under dual limitation of glucose and oxygen |
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Authors: | Hans S van der Hoeven Jan C Gottschal |
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Institution: | Laboratory for Oral Microbiology, Medical Faculty, University of Nijmegen, Nijmegen, The Netherlands;Laboratory for Microbiology, State University Groningen, Haren, The Netherlands |
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Abstract: | Abstract Streptococcus mutans and Actinomyces viscosus are among the dominant species in human dental plaque. In their natural environment, carbohydrate- and oxygen-limited conditions are likely to occur frequently. Therfore, mixed cultures of the 2 species were studied under dual limitation of glucose and oxygen. Over a wide range of oxygen-supply rates, coexistence of A. viscosus and S. mutans was observed, within this range A. viscosus increased almost linearly with oxygen supply. A mathematical model based on Monod-type type kinetics and accounting for uncompetitive inhibition of growth by oxygen was developed to simulate these mixed cultures. The model predicted coexistence over a fairly large range of aeration rates. This finding, in combination with the results of the chemostat experiments, led to the conclusion that coexistence of the two species |
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Keywords: | Mixed culture Coexistence Actinomyces viscosus Streptococcus mutans Oxygen consumption Mathematical model |
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