Effects of light and nitrogen limitation on the cell cycle of the dinoflagellate Amphidinium carteri |
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Authors: | Olson Robert J; Chisholm Sallie W |
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Institution: | Ralph M.Parsons Laboratory 48425 Cambridge, MA 02139, USA
1Present address: Woods Hole Oceanographic Institution, Biology Department Woods Hole, MA 02543, USA |
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Abstract: | Cell cycle phase durations of cultures of Amphidinium carteriin light- or nitrogen-limited balanced growth were determinedusing flow cytometry. For both types of growth rate limitation,the increases in generation time caused by increasing degreesof limitation were due solely to expansion of the G1 phase ofthe cell cycle. The durations of the S and G2 + M phases wereindependent of growth rate. Furthermore, when cells were deprivedcompletely of light and nitrogen, they arrested in the G1 phaseof the cell cycle. The results indicate that light- and nitrogen-dependentprocesses are heavily concentrated in the early part of thecell cycle, while DNA replication and cell division, once initiated,are independent of light or nitrogen supply. |
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