一种快速辨别糙皮侧耳和肺形侧耳的方法

目的：建立一种快速、经济的方法辨别糙皮侧耳（P.ostreatus）和肺形侧耳（P.pulmonarius）。方法：在GenBank下载糙皮侧耳和肺形侧耳的ITS序列,经ClustalX序列比对,利用Primer 3设计特异引物组合1F（5′-GATAGATCTGTGAAGTCGTC-3′）、1R（5′-TCACAATTGGAAAGAAACC-3′）和2R（5′-TGCGTGCTATTGATGAGTGA-3′）,最后经PCR扩增和琼脂糖凝胶电泳检测。结果：5个糙皮侧耳菌株均能得到2条带,分别为342bp和459bp。4个肺形侧耳菌株均能扩增到一条446bp的片段。结论：该组引物适合辨别糙皮侧耳和肺形侧耳。

A Rapid Method for Distinguishing between Pleurotus ostreatus and Pleurotus puhnonarius

Objective: To set up a rapid and economic method for distinguishing Pleurotus ostreatus and Pleurotus pulmonarius. Method: The Internal Transcribed Spacer sequences of P. ostreatus and P. puhnonarius were downloaded from GenBank, aligned by ClustalX, and then the specific primers, 1F (5'-GATAGATCTGTGAAGTCGTC-3'), 1R (5'-TCACAATTGGAAAGAAACC-3') and 2R (5'-TGCGTGCTATT-GATGAGTGA-3'), were designed by Primer 3. After the PCR amplification, it was detected by agarose gel electrophoresis. Result: Amplified with this set of primers, there were two bands, 342bp and 459bp, in all the five strain of P. ostreatus, and a 446bp band in all the four strain of P. pulmonarius . Conclusion: This set of primers was suit for distinguishing between P. ostreatus and P. pulmonarius.