Effect of caffeine on the post-thaw motility of buffalo spermatozoa |
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Authors: | Fattouh El-S M Abdou M S |
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Institution: | Department of Theriogenology Faculty of Veterinary Medicine Cairo University Giza-12211, Egypt. |
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Abstract: | Buffalo semen was diluted (1:2) with lactose diluent containing caffeine (2, 4 and 6 mM). Diluted semen samples were frozen in a pellet form (0.15 ml), thawed 24 h after freezing in 2.9% sodium citrate for 30 sec and incubated at 37 degrees C for 3 h. Addition of caffeine to diluted buffalo semen before freezing resulted in a significant increase in the post-thaw motility of spermatozoa over the 3-h incubation period. When caffeine was added to the thawing medium, the post-thaw motility was further improved. Thus, the increase in motility due to caffiene treatment was even more pronounced than in samples treated with caffiene before freezing. |
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