Deoxynucleotide sequence of an insect cDNA codes for an unreported member of the Chironomus thummi globin family |
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| Authors: | D A Saffarini P M Trewitt M Castro P J Wejksnora G Bergtrom |
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| Affiliation: | 1. University of Wisconsin-Milwaukee, Dept. of Biological Sciences, Milwaukee, WI 53201 USA;2. OCS Laboratories, Denton, TX 76202 USA;1. Dipartimento di Scienze Biochimiche “A. Rossi Fanelli”, Sapienza, Università di Roma, P.le A. Moro 5, 00185, Rome, Italy;2. Institute of Molecular Biology and Pathology, National Research Council, P.le A. Moro 5, 00185, Rome, Italy;3. Center for Life Nanoscience, Istituto Italiano di Tecnologia, 00161, Rome, Italy;1. Institute for Transformative Molecular Medicine, Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, 44106, USA;2. Harrington Discovery Institute, University Hospitals Cleveland Medical Center, Cleveland, OH, 44106, USA;3. Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, 59717, USA |
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| Abstract: | Synthetic oligonucleotides served as probes to isolate insect globin clones from a Chironomus thummi cDNA bank. The cDNA insert of one clone (pC-S9) was completely sequenced by the dideoxy termination procedure. Beginning at the 5' end of the coding region, the 584 base pair sequence encodes most of an N-terminal hydrophobic signal sequence and the complete sequence for a mature secreted globin, and contains a polyadenylation recognition site 3' to an appropriate stop codon. The inferred amino acid sequence is that of an unreported variant of hemoglobin VIIB. Based on the number of differences between Hb VIIB chains, the pC-S9 gene has been evolutionarily independent longer than the other (two) members of the globin VIIB subfamily. |
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