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Haemophilus influenzae and Vibrio cholerae genes for mutH are able to fully complement a mutH defect in Escherichia coli |
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| Authors: | Friedhoff Peter Sheybani Babak Thomas Evangelos Merz Christian Pingoud Alfred |
| Institution: | 1. Department of Microbiology, Peking University Health Science Center, Beijing 100083, China;2. Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Canada AB T2N 4N1;3. Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Canada AB T2N 4N1;4. Cancer Research Center, University of Missouri at Columbia, Columbia, MO 65211, USA;1. College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, China;2. Department of Nutrition and Food Science, University of Maryland, College Park, MD, USA;3. Division of Microbiological Studies, Office of Plant and Dairy Foods, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD, USA;4. Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, US Food and Drug Administration, Laurel, MD, USA |
| Abstract: | MutH, MutL and MutS are essential components of the mismatch repair system in Escherichia coli. Whereas mutS and mutL genes are found in most organisms, the mutH gene is limited to some proteobacteria. We show here that the cloned genes of MutH from Vibrio cholerae and Haemophilus influenzae are able to fully complement a mutH defect in E. coli. Moreover, the purified proteins were shown to be dam methylation sensitive endonucleases, which can be activated by the E. coli MutL protein. These results allow to narrow down regions that are important for the interaction of MutH with MutL. |
| Keywords: | Mismatch repair Endonuclease Genetic complementation Vibrio cholerae Haemophilus influenzae |
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