Affinity of phosphatidylglycerophosphate synthetase for the liponucleotides in Bacillus subtilis |
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| Authors: | Jean-Luc Gaillard Bernard Lubochinsky Daniel Rigomier |
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| Affiliation: | Laboratoire de Biochimie Cellulaire, Universitéde Poitiers, CNRS: LA 290 Biomembranes, 40 avenue du Recteur Pineau, 86022 Poitiers Cedex, France |
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| Abstract: | Abstract In Bacillus subtilis , the synthesis of phosphatidylglycerol, the more reactive phospholipid, was investigated in vitro. The phosphatidylglycerophosphate synthetase was exclusively localized in the membrane fraction. Three phospholipids (phosphatidylglycerophosphate, phosphatidylglycerol and diphosphatidylglycerol) were synthesized by this fraction. Exogenous CDP-diglyceride and dCDP-diglyceride were substrates with the same K m (0.11 mM). In coupled system with CDP-diglyceride synthetase, endogenous dCDP-diglyceride was a less effective substrate than CDP-diglyceride. |
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| Keywords: | Phospholipid synthesis phosphatidylglycerophosphate synthetase liponucleotides regulation Bacillus subtilis |
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