Phorbol 12-myristate 13-acetate activates rabbit neutrophils without an apparent rise in the level of intracellular free calcium |
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| Authors: | R I Sha'afi J R White T F Molski J Shefcyk M Volpi P H Naccache M B Feinstein |
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| Institution: | 1. Department of Physiology, University of Connecticut Health Center, Farmington, CT 06032 USA;2. Department of Pathology, University of Connecticut Health Center, Farmington, CT 06032 USA;3. Department of Pharmacology, University of Connecticut Health Center, Farmington, CT 06032 USA |
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| Abstract: | The addition of low concentrations of phorbol 12-myristate 13-acetate to rabbit neutrophils induces cell aggregation, degranulation, increased oxygen consumption and an increase in the amount of actin associated with the cytoskeleton without a rise in the level of intracellular free calcium as measured using the fluorescent probe quin-2. The ability of phorbol 12-myristate 13-acetate to initiate neutrophil responses similar to those produced by the chemotactic factor without causing a rise in the level of intracellular free calcium suggests two possibilities; that there is a second messenger in addition to calcium or that it activates the cells at a point distal to calcium mobilization. The possible role of diacylglycerol in neutrophil activation is discussed. |
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