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Expression of human interleukin-2 from native and synthetic genes inE. coli: No correlation between major codon bias and high level expression |
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| Authors: | Hee-Won Lee Jae-Hoon Joo Seongman Kang In-Sung Song Jong-Bum Kwon Moon H. Han Doe Sun Na |
| Affiliation: | (1) Department of Biochemistry, College of Medicine, University of Ulsan, 388-1 Pungnap-dong, Songpa-gu, 138-040 Seoul, Korea;(2) Asan Institute for Life Sciences, College of Medicine, University of Ulsan, 388-1 Pungnap-dong, Songpa-gu, 138-040 Seoul, Korea;(3) Genetic Engineering Institute, Korea Institute of Science and Technology, San-1 Uheon-dong, Yuseong-gu, 305-333 Daejun, Korea |
| Abstract: | Summary Expression of human IL-2 from the cDNA and synthetic gene was compared using theE. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system. |
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