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Efficient in vitro propagation of Agave parrasana Berger
Authors:Santacruz-Ruvalcaba  Fernando  Gutiérrez-Pulido  Humberto  Rodríguez-Garay  Benjamin
Institution:1.Centro de Investigación y Asistencia en Tecnología y Dise?o del Estado de Jalisco, A.C., División de Micropropagación y Mejoramiento Genético Vegetal, Av. Normalistas 800 S.H., Guadalajara, Jalisco, 44270, México
;2.Centro Universitario de Ciencias Exactas e Ingeniería, Departamento de Matemáticas, Universidad de Guadalajara, Corregidora No. 500, Guadalajara, Jalisco, 44840, México
;
Abstract:An efficient method for the in vitro propagation of Agave parrasana Berger, an important ornamental plant species native to the state of Coahuila, México, was developed. Proliferation of good quality shoots was achieved on agar-solidified basal MS medium supplemented with L2 vitamins and 13.3 μM benzyladenine. Rooting was successful in the basal medium with no growth regulators; however, a light intensity of 100 μmol m-2 s-1 was found to promote better rooting than 25 μmol m-2 s-1. This revised version was published online in June 2006 with corrections to the Cover Date.
Keywords:Agavaceae  light intensity  response surface  tissue culture
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