A murine monoclonal antibody directed against a yeast cell wall glycoprotein antigen of the yeast genus Saccharomyces |
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| Authors: | Michael Brö ker,Hans-Peter Harthus,Roger M.R. Barnes |
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| Affiliation: | Research Laboratories of Behringwerke AG, Post Box 1140, D-35001 Marburg, FRG; Department of Immunology, Royal Liverpool University Hospital, Post Box 147, Liverpool L69 3BX, UK |
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| Abstract: | Abstract Murine monoclonal antibodies (mAbs) were selected against a cell wall glycoprotein of Saccharomyces cerevisiae . One of the mAbs (92-276/018) specifically identified S. cerevisiae and the sibling species S. paradoxus, S. pastorianus and S. bayanus in immunofluorescence studies and immunoblot analyses, while no other yeast genera except Saccharomyces were recognized. Further analysis indicated that the mAb 92-276/018 reacts with an epitope in the carbohydrate chain of the cell wall glycoproteins. |
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| Keywords: | Saccharomyces cerevisiae Monoclonal antibody Cell wall glycoprotein Immunoblot Immunofluorescence |
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