A filtration, incubation and staining reactor including a new protocol for FISH |
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Authors: | Poschen Lothar Klauth Peter Groeneweg Joost Wilhelm Ralf |
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Institution: | Forschungszentrum Jülich, Institut für Chemie und Dynamik der Geosph?re (ICG), Institut IV: Agrosph?re, Germany. l.poschen@fz-juelich.de |
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Abstract: | A newly developed device for performing fluorescence in situ hybridization (FISH) is described. An adapted procedure was compared with two typical FISH protocols. Tests were performed with Pseudomonas cells and the gene probe EUB338. With the novel procedure, we obtained a better recovery of cells and less variability in results. |
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