Ultramicroassay of hydrogen peroxide-producing oxidases |
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| Authors: | M Le Hir |
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| Affiliation: | Medizinische Universitäts-Poliklinik Basel, Hebelstrasse 20, 4031 Basel, Switzerland |
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| Abstract: | 3-Amino 1,2,4-triazole inhibits catalase irreversibly in the presence of hydrogen peroxide produced by urate oxidase and d-amino acid oxidase. Linearity can be obtained between the inhibition of catalase and the activity of the oxidases. A few microunits urate oxidase and d-amino acid oxidase, corresponding to less than 1 μg frozen-dried rat liver and kidney, respectively, can be determined by the simple assay of the remaining catalatic activity. |
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