Kinetics of ligand binding to a cluster of membrane-associated receptors |
| |
Authors: | Andrew A. Potanin V. Vladyslav Olga S. Belokoneva Frederick W. Wiegel |
| |
Affiliation: | (1) Department of Chemistry, Moscow State University, Russia;(2) Belozersky Institute, Moscow State University, Russia;(3) Institute of Chemical Physics, Russian Academy of Science, Moscow, Russia;(4) Center for Theoretical Physics, Twente University, The Netherlands;(5) Department of Chemical Engineering, Engineering Quad., Princeton University, 08544-5263 Princeton, NJ, USA |
| |
Abstract: | The process of ligand binding to a cluster of membrane-associated receptors is examined theoretically. The theoretical model proposed involves the diffusion of ligands from the solution to the disc-like cluster of receptors on the surface of the spherical cell. When the ligand hits the internal part of the disc-like cluster, it begins to move laterally until it leaves the disc through its outer surface or is bound by one of the receptors inside the disc. If the ligand leaves the cluster, it returns to the solution and hits the disc again after a certain period, etc. According to our model the transition from a diffusion-limited to a reaction-limited process of binding is determined by the dimensionless parameter Dtc/a2, where D is the lateral diffusion coefficient,tc is the characteristic time of reaction, anda is the radius of the disc-like cluster. The forward rate constantkf turns out to be a function of . Comparing the results of our calculations ofkf with some experimental data we found that agreement is achieved at high , i.e. the process of ligand binding by clustered receptors is predominantly reaction-limited. |
| |
Keywords: | Chemoreception Diffusion of ligands Receptors' cluster |
本文献已被 SpringerLink 等数据库收录! |
|