胃癌与幽门螺杆菌cagA、hrgA基因相关性的研究

目的:探讨幽门螺杆菌(Hpylori)菌株中cagA和hrgA基因对胃癌的致病作用及其检测的意义。方法:胃癌及消化性溃疡术后切除标本,组织学检查,快速尿素酶法和PCR检测。结果:40例标本经组织学检查24例为胃腺癌,2例为胃黏膜相关淋巴样组织(MALT)瘤,14例为消化性溃疡。经快速尿素酶法检测,胃腺癌中,12例H pylori( ),消化性溃疡中,12例H pylori( )。经PCR检测,胃腺癌中,18例hrgA( ),6例hrgA(-),20例cagA( ),4例cagA(-);消化性溃疡中,6例hrgA( ),8例hrgA(-),12例cagA( ),2例cagA(-)。结论:H pylori感染与胃癌的发生有密切关系。PCR检测较快速尿素酶法准确。检测cagA和hrgA基因对了解Hpylori菌株的致病性、估计疾病程度、了解病变预后及临床治疗都具有重要意义。

Correlative Study on CagA and HrgA Genes of H Pylori in Gastric Carcinoma

Objective:To investigate the role of hrgA and cagA genes of H.Pylori causing diseases and the value of detection in gastric carcinoma.Methods:Gastric biopsy specimens from 26 patients with gastric carcinoma and 14 with peptic ulcer were obtained,and were performed histopathological examination,rapid urease test and polymerase chain reaction (PGR).Results: Among the detected 26 gastric carcinoma cases,24 cases were gastric adenocarcinoma and 2 cases were gastric MALT Iymphoma. H pylori was present in 12 of 24 cases with gastric adenocarcinoma and in 12 of 14 cases with peptic ulcer by rapid urease test.Of the 24 cases with gastric adenocarcinoma,18 were hrgA positive and 20 were cagA positive;of the 14 cases with peptic ulcer,6 were hrgA positive and 12.was cagA positive by PCR.Conclusion:H.pylori could cause gastric carcinoma,and PGR was more reliable than the urease methods to detect it.It was very important to detect cagA and hrgA of H.pylori for the realization of their role in causing disease,assessing the damage,previewing the disease and choosing clinical treatment.