Oxidation of hydroxylamine to nitrite catalyzed by hydroxylamine oxidoreductase purified fromNitrosomonas europaea |
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Authors: | Tateo Yamanaka Yasuo Sakano |
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Institution: | (1) Department of Biology, Faculty of Science, Osaka University, 560 Toyonaka, Osaka, Japan;(2) Biochemical Research Laboratory, Sun Star Inc., 569 Takatsuki, Osaka, Japan |
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Abstract: | The oxidation of hydroxylamine to nitrite, which had been catalyzed by hydroxylamine oxidoreductase purified fromNitrosomonas europaea, was studied. The enzyme oxidized hydroxylamine almost completely to nitrite under aerobic conditions if sufficient amount
of cytochromec or ferricyanide was added and the reaction was performed in phosphate buffer. Even under anaerobic conditions, hydroxylamine
was oxidized to nitrite by the enzyme, but nitrite, once formed, disappeared when the reaction was continued for more than
several minutes. |
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Keywords: | |
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