Genetically modified coffee plants expressing the Bacillus thuringiensis cry1Ac gene for resistance to leaf miner |
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| Authors: | T Leroy A -M Henry M Royer I Altosaar R Frutos D Duris R Philippe |
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| Institution: | (1) CIRAD-CP, avenue Agropolis, B.P. 5035 34032 Montpellier Cedex 01, France e-mail: thierry.leroy@cirad.fr, FR;(2) CIRAD, avenue Agropolis, B.P. 5035 34032 Montpellier Cedex 01, France, FR;(3) University of Ottawa, 40 Marie Curie, Ottawa ON K1N6N5, Canada, CA |
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| Abstract: | A synthetic version of the cry1Ac gene of Bacillus thuringiensis has been used for the transformation of coffee species (Coffea canephora and C. arabica) to confer resistance to an important pest, the coffee leaf miner (Perileucoptera coffeella and other Leucoptera spp). Somatic embryos were co-cultivated with the LBA4404 strain of Agrobacterium tumefaciens containing the cry1Ac gene. More than 100 transformed plants from independent transformation events were obtained for each coffee genotype.
The integration and expression of the cry1Ac gene was studied, and effective resistance of transgenic plants against leaf miner was verified in bioassays with the
insects. These plants could represent a good opportunity to analyse the impact of genetic engineering of perennial crops for
sustainable resistance to an obligate endocarpic pest using a B. thuringiensis insecticidal protein.
Received: 7April 1999 / Revision received: 20 July 1999 / Accepted: 22 July 1999 |
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| Keywords: | cry1Ac gene Coffea spp Genetic transformation Perileucoptera coffeella Leucoptera spp |
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