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The recognition of abnormal sex chromosome constitution by HLA-restricted anti-H-Y cytotoxic T cells and antibody
Authors:Dr Els Goulmy  Aad van Leeuwen  Els Blokland  Eva S Sachs  Joep P M Geraedts
Institution:1. Department of Immunohaematology, University Hospital Leiden, Rijnsburgerweg 10, 2333, AA Leiden, The Netherlands
2. Department of Clinical Genetics and Department of Pediatrics, University Hospital and Sophia Childrens Hospital, Rotterdam, The Netherlands
3. Department of Human Genetics, State University Leiden, The Netherlands
Abstract:Using the cell-mediated lympholysis (CML) technique, we studied lymphocytes of six individuals with discrepancies between the karyotypic and phenotypic sex. Two sets of cytotoxic T cells (CTLs) obtained from two multitransfused female aplastic anemia patients were used as typing reagents. These cells were previously shown to kill allogeneic target cells from HLA-A2- or B7-positive male donors. An antiserum obtained from one of the patients likewise killed HLA-A2 male lymphocytes. The six patients studied were selected for the required antigens. Positive reactions were obtained with lymphocytes from a 46, XY woman with pure gonadal dysgenesis and a 45, XO male. Target cells of the mother of the latter patient were also lysed. One individual with a 45, XO/46, X, del(Y)? karyotype was weakly positive, while three 46, XX males were completely negative. The reactivity of the HLA-A2-restricted H-Y-specific antibody showed the same discriminatory patterns. The results obtained by theHLA-restricted CTLs as well as by the antiserum did not correlate with the presence of testes as is the case in a different test system for the serologically detectable male (SDM) antigen in man. On the other hand, there was a correlation with the presence of cytologically detectable Y-chromosome material in five of the six individuals studied. The HLA-restricted CTLs and the antibody might recognize the classical transplantation antigen H-Y.
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