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New methodology for fungal screening: Xylanolytic enzymes
Authors:E. Curotto  C. Aguirre  M. Concha  A. Nazal  V. Campos  E. Esposito  R. Angelo  A. M. F. Milagres  N. Duran
Affiliation:(1) Inst. Quim. and Biol., Universidad Catolica de Valparaiso, 4059 Casilla, Valparaiso, Chile;(2) Biotechnol. Center, Lorena, S.P., Brazil;(3) Inst. Quim., Biol. Chem. Lab., Universidade Estadual de Campinas, C.P.6154, CEP 13081-970 S.P., Brazil
Abstract:Summary The technique for determining extracellular xylanolytic activity consists of growing selected fungi for four days on agar medium containing 1% xylan. A section of the agar is then homogenized in buffer and the filtered solution tested for xylanase activity and other related enzymes.
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