Changes in the lipid physical state in human erythrocyte membranes subjected lead exposure in vitro |
| |
Authors: | Oleksiuk O B Finin V S Slobozhanina E I |
| |
Affiliation: | Institute of Photobiology, National Academy of Sciences of Belarus, ul. Akademicheskaya 27, Minsk, 220072 Belarus. |
| |
Abstract: | The effect of lead acetate on the physical state of membrane lipids in human erythrocytes in vitro was studied using the lipophilic fluorescence probe 1,6-diphenyl-1,3,5-hexatriene and spin probes 16-doxyl-stearate and iminoxyl palmitic acid. It was shown that 2-10 microM lead acetate causes an increase in both intensity and polarization of fluorescence of 1,6-diphenyl-1,3,5-hexatriene, indicating changes in the microviscosity of the lipid bilayer of erythrocyte membranes. Judging from the parameters of EPR spectra of 16-doxyl stearate and iminoxyl palmitic acid incorporated into erythrocyte membranes, 2-10 microM lead acetate increases the heterogeneity of the lipid bilayer in surface and deep hydrophobic layers of the erythrocyte membrane. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|