| Abstract: | The effect of defocusing on the electron microscopic image of the extended particle of pyocin R1 was examined with the aid of optical diffraction. The results show that the through-focus image of the cross-striated particle changed to a fishbone-like image with a little under-focusing, which had been considered to be a transitional state during the sheath contraction of phage G. In the optical diffraction pattern of the defocused image, a strong meridional reflection corresponding to the distance of cross-striations in the through-focus image almost disappeared, supporting the change to the fishbone-like image on defocusing. |
