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植物生长调节剂对黑木相思优树腋芽增殖及生根的影响
引用本文:黄烈健,王鸿,胡峰,施琼.植物生长调节剂对黑木相思优树腋芽增殖及生根的影响[J].热带亚热带植物学报,2015,23(6):669-674.
作者姓名:黄烈健  王鸿  胡峰  施琼
作者单位:中国林业科学研究院热带林业研究所, 广州 510520,中国林业科学研究院热带林业研究所, 广州 510520,广东省农业科学院作物研究所, 广州 510640,中国林业科学研究院热带林业研究所, 广州 510520
基金项目:国家 "十二五"科技支撑计划项目(2012BAD01B0402)资助
摘    要:为建立黑木相思(Acacia melanoxylon)快繁技术体系,以含1个腋芽的无菌茎段为材料,研究了植物生长调节剂对其增殖和生根的影响。结果表明,6-BA 极易诱导黑木相思愈伤组织形成,但芽长势较差,不利于腋芽增殖体系的建立。而生长素既能诱导黑木相思生根,又能诱导腋芽增殖;将无菌茎段接入MS+IAA 0.5 mg L-1+IBA 0.5 mg L-1培养基中培养20 d的生根率为98.41%,培养40 d的腋芽增殖倍数为2.36,单株繁殖系数为6.57。这是首次成功建立高效、简便的生根和增殖同步发生的黑木相思直接器官发生途径的组培技术体系。

关 键 词:黑木相思  腋芽增殖  生根  直接器官发生  间接器官发生
收稿时间:2015/3/23 0:00:00
修稿时间:2015/4/30 0:00:00

Effects of Plant Growth Regulators on Axillary Bud Proliferation and Rooting of Acacia melanoxylon Elite Tree
HUANG Lie-jian,WANG Hong,HU Feng and SHI Qiong.Effects of Plant Growth Regulators on Axillary Bud Proliferation and Rooting of Acacia melanoxylon Elite Tree[J].Journal of Tropical and Subtropical Botany,2015,23(6):669-674.
Authors:HUANG Lie-jian  WANG Hong  HU Feng and SHI Qiong
Institution:Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China,Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China,Crop Research Institute, Guangdong Academy of Agricultural Science, Guangzhou 510640, China and Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China
Abstract:In order to establish rapid propagation system of Acacia melanoxylon, the effects of plant growth regulators on its axillary bud multiplication and rooting were studied by using sterile stem segments with one axillary bud as explants. The results showed that the calli of A. melanoxylon were easily induced and bud growth was poor by 6-BA, indicating the disadvantage of axillary bud proliferation. The proliferation and rooting of Acacia melanoxylon axillary buds could be induced by auxin. The stem sections were cultured on MS+IAA 0.5 mg L-1+IBA 0.5 mg L-1 medium, rooting rate was 98.41% cultured for 20 d, and cultured for 40 d, the axillary bud multiplication and propagation coefficient were 2.36 and 6.57 per plantlet, respectively. It was successfully established the direct organogenesis technology system of A. melanoxylon for the first time with the advantages of high efficient, easy rooting and proliferation occur synchronous.
Keywords:Acacia melanoxylon  Axillary bud proliferation  Rooting  Direct organogenesis  Indirect organogenesis
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