Somatic embryogenesis in Ranunculus asiaticus L. hybr. thalamus cultivated in vitro |
| |
Authors: | Margherita Beruto Pierre Debergh |
| |
Affiliation: | (1) Istituto Regionale per la Floricoltura, Via Carducci 12, 18038 Sanremo, Italy;(2) Laboratory for Horticultural Science, Coupure Links 653, 9000 Gent, Belgium |
| |
Abstract: | Calli derived from in vitro cultivated thalamus of Ranunculus asiaticus L. were initiated and maintained for 75 days on Murashige & Skoog's medium containing five concentrations of 2,4-d (0.1, 0.2, 0.4, 0.8, 1.6 mg l-1). Embryoid differentiation occurred on calli initiated on 1.6 mg l-1 2,4-d 75 days after subculture onto hormone-free medium. Calli which were initiated and maintained for 75 days on lower 2,4-d concentrations, then transferred to medium without hormones for 75 days, showed the first embryoids one month after further subculture on medium containing 0.05 mg l-1 2,4-d. All the somatic embryos developed into plants, and 96% survived transplantation to in vivo growth conditions. |
| |
Keywords: | callus in vitro culture Ranunculus asiaticus L. somatic embryogenesis thalamus |
本文献已被 SpringerLink 等数据库收录! |
|