Fatty acid composition in native and cultured human endothelial cells |
| |
Authors: | M Lagarde B Sicard M Guichardant O Felisi M Dechavanne |
| |
Institution: | (1) Institut Pasteur, Laboratoire d'Hémobiologie, Faculté de Médecine Alexis Carrel, INSERM U63, 69372 Lyon Cedex 2, France |
| |
Abstract: | Summary Endothelial cells from human umbilical veins were isolated by collagenase treatment. Cells were cultured in the presence of
either 20% fetal bovine serum (FBS) or 20% human serum (HS). At confluency, endothelial cell lipids were labeled with tracer
concentrations of tritiated arachidonic acid, then extracted and separated into lipid subclasses by thin layer chromatography.
The fatty acid composition of each lipid class was determined by glass capillary gas-liquid chromatography analysis and compared
to that of cells freshly isolated from the cord (NC cells). The fatty acid compositions differed only in phospholipids. Polyunsaturated
fatty acids (PFAs), arachidonic, and linoleic acids were depleted in FBS cell phospholipids and replaced by both stearic and
oleic acids. No significant difference could be observed between NC cell and HS cell phospholipids. We conclude that PFAs
might be decreased in FBS cells because of the relative paucity of PFAs in FBS as compared to HS. It seems therefore more
convenient to cultivate endothelial cells in the presence of HS, especially in respect to their phospholipid content of arachidonic
acid, which is the physiological reservoir for prostacyclin synthesis.
This work was supported by a grant from the Délégation Générale à la Recherche Scientifique et Technique, Paris, France (79.7.0091). |
| |
Keywords: | endothelial cells culture media lipid composition |
本文献已被 SpringerLink 等数据库收录! |
|