| 顺铂与小鼠艾氏腹水肝癌细胞膜的相互作用 |
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| 引用本文： | 陈宝卫,王夔.顺铂与小鼠艾氏腹水肝癌细胞膜的相互作用[J].中国生物化学与分子生物学报,1992,8(5):583-586. |
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| 作者姓名： | 陈宝卫 王夔 |
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| 作者单位： | 北京医科大学无机化学教研室，天然药物与仿生药物国家重点实验室 北京 100083，北京 100083 |
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| 基金项目： | 国家自然科学基金，国家教委博士点基金 |
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| 摘 要： | 本文研究了顺铂对小鼠艾氏腹水肝癌细胞膜蛋白内源性荧光的淬灭作用和测定了其在膜上的结合量。结果表明顺铂能与癌细胞膜结合。按存在两类结合部位,得到表观结合常数和结合部位数为: K_1=1.35×10~5L/mol n_1=6.80×10~(-4)mol/g(protein) K_2=2.50×10~3L/mol n_2=1.92×10~(-3)mol/g(protein)
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| 关 键 词： | 顺铂 癌细胞膜 荧光淬灭 |
| 收稿时间： | 1992-10-20 |
Interaction of Cisplatin with Membranes of Rat Tumor Cell |
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| Chen,Bao-wei Wang,Kui.Interaction of Cisplatin with Membranes of Rat Tumor Cell[J].Chinese Journal of Biochemistry and Molecular Biology,1992,8(5):583-586. |
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| Authors: | Chen Bao-wei Wang Kui |
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| Institution: | (Department of Inorganic Chemistry,National Research Laboratories of Natural and Biomimetic Drugs,Beijing Medical University,Beijing 100083 |
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| Abstract: | Quenching effect of cis-DDP on fluorescence of membranes of rat Ehr-lich ascites tumor cell and binding of cis-DDP with the membranes were studied.The results indicate that cis-DDP can react with tumor cell membrane proteins.If the Pt binding sites were grouped into two types, the apparent stability constants (K)and number of binding sites(n) are estimated:K1=1.35*********x105L/mol n1=6.80*********x10-4mol/g (protein) K2=2.50********x103L/mol n2=1.92********x10-3mol/g (protein) |
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| Keywords: | Cisplatin Tumor cell membrane Fluorescence |
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