| 皱皮木瓜愈伤组织诱导与快速繁殖 |
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| 引用本文： | 范小峰,郭小强,肖朝霞,刘灵霞.皱皮木瓜愈伤组织诱导与快速繁殖[J].植物学报,2009,44(6):725-727. |
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| 作者姓名： | 范小峰 郭小强 肖朝霞 刘灵霞 |
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| 作者单位： | 陇东学院生命科学系, 庆阳 745000 |
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| 摘 要： | 以皱皮木瓜(Chaenomeles speciosa)当年生枝条的茎尖和茎段为外植体进行离体培养与快速繁殖。结果表明: 芽萌发及愈伤组织诱导的最佳培养基是MS+2.0 mg.L-1 6-BA+1.0 mg.L-1 NAA; 愈伤组织分化最佳培养基是MS+1.0 mg.L-1 6-BA+0.05 mg.L-1 NAA; 芽增殖最佳培养基是MS+1.0 mg.L-1 6-BA+0.05-0.1 mg.L-1 NAA, 其增殖系数为6.59; 最佳生根培养基是1/2MS+1.0 mg.L-1 IAA, 生根率为86.7%; 采用珍珠岩二步移栽, 成活率可达90%以上。
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| 关 键 词： | 愈伤组织诱导 皱皮木瓜 快速繁殖 |
| 收稿时间： | 2008-12-24 |
| 修稿时间： | 2009-06-22 |
Callus Induction and Rapid Propagation of Chaenomeles speciosa |
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| Xiaofeng Fan,Xiaoqiang Guo,Zhaoxia Xiao,Lingxia Liu.Callus Induction and Rapid Propagation of Chaenomeles speciosa[J].Bulletin of Botany,2009,44(6):725-727. |
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| Authors: | Xiaofeng Fan Xiaoqiang Guo Zhaoxia Xiao Lingxia Liu |
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| Institution: | Department of Life Sciences, Longdong University, Qingyang 745000, China |
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| Abstract: | This experiment used the current stem-segment with shoot apexes and segments as explants in callus induction and rapid propagation of Chaenomeles speciosa. The optimal medium for bud and callus induction was MS+2.0 mg.L-1 6-BA+1.0mg. L-1 NAA, and the optimal propagation medium was MS+1.0 mg.L-1 6-BA+0.05-0.1 mg.L-1 NAA. The proliferation coefficient was 6.59. For callus differentiation, the optimal medium was MS+ 1.0 mg.L-1 6-BA+ 0.05 mg.L-1 NAA, and for rooting, 1/2 MS+1.0 mg. L-1 IAA. The ratio of rooting was 86.7%, and that of survival was more than 90% when small intact plants were transplanted on perlite seedbeds. |
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| Keywords: | , |
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