| 高免疫原性的高压力失活病毒 |
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| 引用本文： | 田少敏 钱建飞. 高免疫原性的高压力失活病毒[J]. Acta biochimica et biophysica Sinica, 1999, 31(3): 334-336 |
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| 作者姓名： | 田少敏 钱建飞 |
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| 作者单位： | [1]中国科学院上海生物化学研究所 [2]江苏省农业科学院畜牧兽医研究所 |
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| 摘 要： | 以鸡传染性法氏囊病病毒（ＩＢＤＶ）强毒株为对象研究了高压力对ＩＢＤＶ活性的影响。以鸡胚成纤维细胞和ＳＰＦ雏鸡为模型的研究表明，ＩＢＤＶ的感染活性随压力和作用时间的增加而降低，直至完全丧失，但高压力失活的ＩＢＤＶ可有效诱导雏鸡血清抗体大量产生，对雏鸡的保护作用达１００％，且无发病现象，说明ＩＢＤＶ经一定高压力条件处理后仍具有高免疫原性，即具备了疫苗的特性，内源荧光光谱、内源荧光偏振，散射光及ｂｉｓ－
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| 关 键 词： | 高压力 失活 鸡法氏囊病病毒 免疫原性 疫苗 |
High Immunogenicity of the Pressure inactivated Virus |
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| TIAN Shao Min,QIAN Jian Fei,SHAO Guo Qing and RUAN Kang Cheng. High Immunogenicity of the Pressure inactivated Virus[J]. Acta biochimica et biophysica Sinica, 1999, 31(3): 334-336 |
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| Authors: | TIAN Shao Min QIAN Jian Fei SHAO Guo Qing RUAN Kang Cheng |
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| Affiliation: | TIAN Shao Min,QIAN Jian Fei,SHAO Guo Qing and RUAN Kang Cheng * |
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| Abstract: | Effects of high hydrostatic pressure on the activity of infectious bursal disease virus(IBDV) was described. The infectivity of IBDV decreased with the increasing of pressure and pressurizing time and may be completely lost, indicated by the tests with chicken embryo fibrablast cell and young chicken as models. How ever, pressure inactivated IBDV still remained high immunogenicity and might induce high levels of protecting serum antibody. Fluorescence spectroscopy showed obvious differences between the structures of native and pressurized IBDV, similar with the dissociation reassociation of oligomeric proteins under high pressure. The changes of pressurized IBDV were also directly observed with electron microscopy. These results suggest that pressure inactivated IBDV may be used as a vaccine. |
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| Keywords: | High hydrostatic pressure infectious bursal disease virus (IBDV) immunogenicity vaccine |
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