蛋白激酶C抑制剂对U937细胞清道夫受体功能的影响

为了解细胞内蛋白质磷酸化水平对清道夫受体功能的影响，用蛋白激酶Ｃ抑掉剂形孢菌素（ｓｔａｕｒｏｓｐｏｒｉｎｅ，ＳＴＡ）处理人Ｕ９３７细胞，分别测定对照组和处理组细胞对碘标记的氧化低密度脂蛋白（＾１２５Ｉ）ｏｘ－ＬＤＬ的降解，结合，细胞表面受体复合物的内移以及细胞内脂质蓄积的程度，并利用放射自显影方法观察药物对细胞表面受体表达的影响，结果发现ＳＴＡ可以促进细胞结合（＾１２５Ｉ）ｏｘ－ＬＤＬ增加细胞表面

Effect of Protein Kinase C Inhibitor on Scavenger Receptor in Human U937 Macrophage like Cells

In order to investigate effects of cell protein phosphorylation on scavenger receptor, human U937 macrophage like cells were treated with protein kinase C inhibitor staurosporine, then the cells were incubated with 125 I]ox LDL or ox LDL ,and the cellular degradation of 125 I]ox LDL, its binding to receptor and the internalization of cell surface ox LDL receptor complex as well as the accumulation of lipids within cells were measured separately. Moreover, the effects of the drug on expression of cell surface receptor were observed by means of autoradiography. The results indicated that staurosporine could enhance U937 cells to bind lipids and stimulate scavenger receptor expression, and could reduce degradation of lipids by U937 cells and the accumulation of cholesterol within the cells. It suggests that the function of scavenger receptors may be correlated with cell protein phorsphorylation.