酵母双杂交技术及其在蛋白质组研究中的应用

蛋白质组学是后基因组时代出现的一个新兴的研究领域，它的主要任务是识别鉴定细胞，组织或机体的全部蛋白质，并分析蛋白质的功能及其模式。因此，揭示蛋白质组中蛋白质间的相互作用关系也是蛋白质组学的重要内容之一。酵母双杂交技术是用来检测蛋白质间是否相互作用的一个非常有效的手段，该技术在酵母蛋白质组研究中的初步成功应用，表明它有望在人类蛋白质且研究中发挥重要作用。

Yeast Two hybrid System and Its Application on Proteomics

Proteomics is a new research area of the post genomic era that aims at the analysis and identification of the entire proteins present in the cell, tissue or organism, and of the functions and the linkages of these proteins. Protein protein interactions are characteristic of cellular activities and of course an important part of proteomics. One of the effective techniques to detect protein protein interaction is yeast two hybrid system. This system was successfully employed to analyze the protein linkage map of the yeast mRNA splicing machinery, which boded well for its application on human proteome research.