Expression of aChlamydia anticarbohydrate single-chain antibody as a maltose binding fusion protein |
| |
Authors: | Douglas P. Malinowski Mary Gourley Susan Edelstein Robert E. Pearson |
| |
Affiliation: | (1) Department of Molecular Biology, Becton Dickinson Research Center, PO Box 12016, 27709 Research Triangle Park, NC;(2) Glaxo Inc., Five Moore Drive, 27709 Research Triangle Park, NC;(3) National Institute of Environmental Health Sciences, 111 Alexander Dr., 27709 Research Triangle Park, NC |
| |
Abstract: | A single-chain antibody fragment has been constructed for an antibody that binds to theChlamydia specific carbohydrate structure of the lipopolysaccharide. Single-chain protein was expressed and secreted into the periplasmic space ofE. coli as a fusion protein with the maltose binding protein. The fusion protein was purified in one step by virtue of its ability to bind to maltose. In a sandwich ELISA, the eluted protein boundChlamydia lipopolysaccharide, which demonstrates that the single-chain protein domain will function as part of a fusion protein. The expression of maltose binding fusion proteins into the periplasmic space could be used for production of other single-chain antibodies or protein fragments requiring appropriate folding and disulfide bond formation. |
| |
Keywords: | Single chain antibody maltose fusion protein Chlamydia LPS |
本文献已被 SpringerLink 等数据库收录! |
|