Immobilization of wood-rotting fungi laccases on modified cellulose and acrylic carriers |
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Authors: | Abdulkareem JH Al-Adhami Jolanta Bryjak Beata Greb-Markiewicz Wanda Peczyska-Czoch |
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Institution: | Abdulkareem J.H. Al-Adhami, Jolanta Bryjak, Beata Greb-Markiewicz,Wanda Peczyńska-Czoch, |
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Abstract: | Extracellular laccases produced by three different wood-rotting fungi, Cerrena unicolor, Heterobasidion annosum and Trametes versicolor, were immobilized via covalent bonds formation on DEAE-Granocel 500, CM-Granocel 500, and acrylic carriers. Out of the tested carriers, only the DEAE-Granocel 500, which was activated by divinyl sulphone appeared to be a suitable matrix for the expression of enzymic activity. Only one laccase of all the tested enzymes produced by C. unicolor showed the best binding to the carrier and a satisfactory enzymic activity. The immobilized laccase exhibited the highest enzymic activity at pH 5.2 and it was more resistant to thermal denaturation than the native enzyme. At 90 °C, it retained 75% activity compared to the free enzyme. It was also more stable during storage at 4 °C: after 4 months the immobilized laccase retained 98% of initial activity. Immobilized C. unicolor laccase was active in 10–60% concentration of methanol, acetone, isopropanol or acetonitrile. The best enzymic activity was observed in 20% solution of acetonitrile in buffer. |
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Keywords: | Laccase Wood-rotting fungi Cerrena unicolor Enzyme immobilization |
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