The search for the DNA of the chick embryo fibroblast cytosolic complex |
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Authors: | C Challen D H Adams |
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Institution: | Department of Virology (Annexe), Newcastle upon Tyne, England. |
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Abstract: | 1. Conventional DNA extraction procedures have failed to release free DNA from the chick embryo fibroblast cytosolic DNA-RNA complexes. 2. Free DNA has been released only from the smallest cell cytosol DNA fraction, which is not in the native state associated with RNA: it is very small (of the order of 100 bases) and single stranded. 3. However, phenol extraction does separate complex DNA-associated material from the RNA which has invariably been found to accompany it in all but the smallest fraction (see 2 above). 4. The principal factor preventing DNA release appears to be a massive aggregation of partially purified DNA-associated material. |
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