大鼠脊神经节内交感神经支配的荧光组织化学与HRP示踪观察

本研究应用乙醛酸诱发儿茶酚胺（ＣＡ）荧光技术观察大鼠肾上腺素（ＮＡ）能神经在脊神经节内的分布；并应用ＨＲＰ顺、逆行追踪技术对脊神经节内ＮＡ能神经纤维的起源及其与脊神经节神经元的关系进行了探讨。荧光组织化学观察发现、有些神经节神经元胞体周围分布有带膨体的ＮＡ能神经末梢；有的紧密围绕脊神经节细胞——卫星细胞复合体。颈上交感神经节内注射霍乱毒素Ｂ亚单位结合ＨＲＰ（ＣＢ┐ＨＲＰ），在同侧Ｃ３～６节段脊神经节内可见标记的点状纤维末梢紧邻于节细胞旁。Ｔ１１～Ｌ２节段脊神经节内注射ＨＲＰ后，在同侧椎旁交感链（Ｔ９～Ｌ１）内可见标记的交感节后神经元胞体。上述实验结果表明，交感节后神经元发出节后纤维可直接到达脊神经节内，与节细胞发生接触。本研究提示、交感神经在脊神经节水平可能参与躯体初级传入信息的调制

FLUORESCENCE HISTOCHEMICAL AND HRP TRACE OBSERVATION ON THE SYMPATHETIC NEURAL INNERVATION IN SPINAL GANGLION OF THE RAT

In this paper we have observed the distribution of noradrenergic nerves in spinal ganglion of the rat by using glyoxylic acid induced fluorescence histochemical technique. The origin of noradrenergic nerve fibers in spinal ganglion as well as the interaction between spinal ganglion neurons and noradrenergic nerves were also studied. The results of fluorescence histochemistry exhibited that there were noradrenergic fibers with varicosities around some spinal ganglia neurons, some of which closely encompassed the spinal ganglia neuron__satellite cell complexes. The labelled specked nervous fibers close to ganglia cells were observed in ipsilateral fragments of cervical ganglions (C3 C6) after injecting CB HRP in superior cervical ganglion. After injecting HRP in T11 12 fragments of spinal ganglion, the labelled postsympathetic neurons were seen in ipsilateral sympathetic chain (T9 L1). The above results indicated that the nervous fibers originated from postsympathetic neurons could directly reach the spinal ganglions and could contact with spinal ganglia neurons. It suggested that primary somatic sensory afferent message could be regulated at the level of spinal dorsal root ganglion.