Evaluation of immobilized boronates for studies of adenine and pyridine nucleotide metabolism |
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Authors: | R Alvarez-Gonzalez H Juarez-Salinas E L Jacobson M K Jacobson |
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Institution: | 1. Department of Chemistry, North Texas State University/Texas College of Osteopathic Medicine, Denton, Texas 76203, USA;1. Department of Biochemistry, North Texas State University/Texas College of Osteopathic Medicine, Denton, Texas 76203 USA;2. Department of Biology, Texas Woman''s University, Denton, Texas 76204 USA |
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Abstract: | Three different immobilized boronates were compared with respect to their possible utility in studies of adenine and pyridine nucleotide metabolism. These included boronate derivatives of polyacrylamide, Sepharose, and the cation-exchange resin, Bio-Rex 70. The relative binding affinities, binding capacities, and elution properties were compared. Under the conditions utilized, the Sepharose and Bio-Rex 70 derivatives selectively retained nucleotides containing two or more sets of 1,2 cis-diol groups. Since the bulk of cellular nucleic acids contain but a single set of 1,2 cis-diol groups or less, these boronate derivatives are very useful for the isolation of the acid-soluble nucleotides, NAD and diadenosine 5',5"'-tetraphosphate, and for the acid-insoluble polymer, poly(ADP-ribose). The Bio-Rex 70 derivative had a particularly useful combination of binding selectivity, capacity, and elution characteristics. Specific applications of this resin for studies of NAD and poly(ADP-ribose) metabolism are presented. |
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Keywords: | boronyl resins NAD poly(ADP-ribose) mono(ADP-ribose) diadenosine tetraphosphate adenine nucleotides |
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