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Intérêt de la spectrométrie de masse de type MALDI-TOF pour l’identification des champignons filamenteux
Authors:M -É Bougnoux  C Lacroix  N Hassouni  J -L Beretti  B Dauphin
Institution:1.Service de microbiologie, h?pital Necker Enfants Malades,APHP,Paris,France;2.Service de parasitologie-mycologie, h?pital Saint Louis,APHP,Paris,France
Abstract:Identification of filamentous fungi, molds and dermatophytes, is currently based on the morphological study of colonies and therefore the experience of the mycologist. These techniques are not sufficiently precise to distinguish between different species within the same section. Furthermore, identification can be delayed for several weeks due to subcultures on specific media. MALDI-TOF MS allows correct identification of filamentous fungi until the species level in more than 95% of cases in most studies. MALDI-TOF MS is a fast and precise identification technique for filamentous fungi; however most of the different databases need to be further evaluated in routine and completed to broaden the spectrum of species identified.
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