Distribution of tyrosinated and acetylated tubulin in centrioles during mitosis of 3T3 and SV40-3T3 cells |
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Authors: | E E Balashova P G Lokhov V B Bystrevskaya |
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Institution: | (1) State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, The Third Military Medical University, 400038 Chongqing, People’s Republic of China;(2) Department of Gynecology and Obstetrics, Daping Hospital, The Third Military Medical University, 400038 Chongqing, People’s Republic of China; |
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Abstract: | We performed a comparative electron microscopic analysis of centriolar and cytoplasmic microtubules stained with antibodies
to acetylated or tyrosinated α-tubulin during the cell cycle of mouse nonmalignant Balb 3T3 (clone A31) and virus-transformed
heteroploid SV40-3T3 cell lines. It was shown that the pattern of centriole immunostaining changed during the cell cycle in
3T3 (A31) cells, but not in tumorigenic SV40-3T3 cells. Remarkable changes in the centriole immunostaining pattern were observed
during interphase-mitosis or mitosis-interphase transitions when the microtubule system and protein organization of centrosomes
underwent drastic rearrangements. A high level of tyrosinated tubulin in centrioles was observed at all stages of the cell
cycle except when entering mitosis, whereas a high level of acetylated tubulin was visualized in centrioles at all stages
of the cell cycle except at the end of mitosis. |
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