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Fine binding specificities to Ascaris suum and Ascaris lumbricoides antigens of the sera from patients of probable visceral larva migrans due to Ascaris suum |
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| Authors: | Haruhiko Maruyama Shinichi Noda Won-Young Choi Nobuo Ohta Yukifumi Nawa |
| Institution: | aDepartment of Parasitology, Miyazaki Medical College, Kiyotake, Miyazaki 889-26, Japan;bDepartment of Medical Zoology, Faculty of Medicine, Kagoshima University, Kagoshima, Japan;cDepartment of Parasitology, Catholic University Medical College, Seoul, Korea;department of Medical Zoology, Nagoya City University, Nagoya, Japan |
| Abstract: | Fine binding specificities to Ascaris suum and A. lumbricoides antigens of the sera from patients with probable visceral larva migrans (VLM) due to A. suum infection were examined. Although multiple-dot enzyme-linked immunosorbent assay (ELISA) was found to be useful for the primary screening of patients, identification of the responsible species was sometimes difficult due to extensive cross reactions with other ascarid parasite antigens. Fine resolution to determine the causative pathogen was obtained by a rather classical Ouchterlony's double immunodiffusion test. The difference in the binding of the patients' sera to A. suum and A. lumbricoides antigens was also demonstrated by an inhibition ELISA. The patients' antibodies bound with higher avidity to the A. suum antigen than to the A. lumbricoides and Toxocara canis antigens. Combination of at least two different immunological assay methods is recommended for the diagnosis of VLM due to ascarid parasites. |
| Keywords: | mmunodiagnosis Visceral larva migrans (VLM) Ascaris suum Ascaris lumbricoides |
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