鸽源H5N1亚型禽流感病毒株的HA和NA基因特征性分析

本研究自行设计合成两对特异性引物，通过RT-PCR扩增出1株鸽源H5N1亚型禽流感病毒血凝素（HA）和神经氨酸酶（NA）两个基因的cDNA片段，将它们成功克隆于pMD18-T载体上，然后进行序列测定。结果表明，HA基因全长1707bp，编码568个氨基酸， HA基因有7个糖基化位点，在裂解位点附近有连续6个碱性氨基酸（R-R-R-K-K-R）的插入，具有高致病性毒株的分子特征。受体结合位点的氨基酸分别为YWIHELY，左侧壁氨基酸为SGVSSA，右侧壁为NGQSGR；NA基因全长1350bp，编码446个氨基酸，NA基因有3个糖基化位点。

Characterization of HA Gene and NA Gene of A pigeon H5N1 Influenza A Virus

RT-PCR were employed to amplify the cDNAs of HA gene and NA gene of A pigeon avian influenza isolate with two pairs of primers. The cDNAs were cloned to pMD18-T and then be sequenced. The results of sequence analysis shows that the cloned HA gene consists of 1707 nucleotides and encodes 568 amino acids residues and contains whole open reading frame. There are 7 potential glycosylation sites and 6 basic amino acids(R-R-R-K-K-R) insert at the cleavage site in HA of the influenza virus isolate which is a symbol of highly pathogenic avian influenza. Amino acids of receptor-binding site are YWIHELY, amino acids of left edge of receptor-binding site are SGVSSA, amino acids of right edge of receptor-binding site are NGQSGR; The cloned NA gene consists of 1350 nucleotides and encodes 446 amino acids residues, there are 3 Potential glycosylation sites in NA of the influenza virus isolate.