Brain elongation of linoleic acid is a negligible source of the arachidonate in brain phospholipids of adult rats |
| |
Authors: | DeMar James C Lee Ho-Joo Ma Kaizong Chang Lisa Bell Jane M Rapoport Stanley I Bazinet Richard P |
| |
Institution: | Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Building 9, Room 1S 126, Bethesda, MD 20892-1582, USA. |
| |
Abstract: | The extent to which the adult brain can derive some of its arachidonic acid (AA) through internalized synthesis from linoleic acid (LA) is uncertain. Thus, we determined for plasma-derived LA in vivo rates for brain incorporation, beta-oxidation, and conversion to AA. Adult male unanesthetized rats, reared on a diet enriched in LA but low in AA, were infused intravenously for 5 min with 1-(14)C]LA. Timed arterial samples were collected until the animals were killed at 5 min and the brain was removed after microwaving. Within plasma lipids, >96% of radioactivity was in the form of unchanged 1-(14)C]LA, but (14)C]AA was insignificant (<0.2%). Eighty-six percent of brain radioactivity at 5 min was present as beta-oxidation products, whereas the remainder was mainly in 'stable' phospholipid or triglyceride as LA or AA (11 and <1%, respectively). Unesterified unlabeled LA rapidly enters brain from plasma, but its incorporation into brain total phospholipid and triglyceride, in the form of synthesized AA, is <1% of the amount that enters the brain. Thus, in rats fed even a diet containing low amounts of AA, the LA that enters brain is largely beta-oxidized, and is not a major source of AA in brain. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|