Separation of mannosylretinylphosphate from dolichylmannosylphosphate by chromatography on columns of DEAE-sephacel |
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| Authors: | W Sasak C S Silverman-Jones L M De Luca |
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| Institution: | National Institutes of Health, Bethesda, Maryland 20014 USA |
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| Abstract: | A one-step column chromatographic procedure on DEAE-Sephacel allows the separation of mannosylretinylphosphate from dolichylmannosylphosphate with minimal breakdown of the mannosylretinylphosphate. Using this procedure, subcellular fractions of rat liver were shown to be active in synthesizing both mannolipids from GDP-14C]mannose in the absence or presence of exogenous retinylphosphate. |
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