Abstract: | Background We developed an efficient in vitro method to differentiate mouse ES cells into the definitive endoderm (DE) and then Pdx1-expressing pancreatic lineages using
mesodermal-derived supporting cells, M15. Using this method, resulting ES cell-derived DE and Pdx1-expressing cells were isolated
by cell sorting, and their gene expression profiles were investigated with DNA microarray. Genes that were specifically expressed
in DE and/or in Pdx1-expressing cells were extracted and their expression patterns in normal embryonic development were studied. |