Two-level factorial screening for influence of temperature, pH, and aeration on production of Serratia marcescens nuclease |
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| Authors: | P K Jepsen E Riise K Biedermann P C Kristensen C Emborg |
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| Institution: | Department of Biotechnology, Technical University of Denmark, Lyngby. |
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| Abstract: | Two high-nuclease-yielding mutants of Serratia marcescens, derived by chemical mutagenesis (W280, W355), and two strains with the pBR322 plasmid 403-SD2, carrying a nuclease gene and a chloramphenicol resistance gene Escherichia coli CSH50(403-SD2) and S. marcescens CH30(403-SD2)] were investigated for nuclease production in a factorial shake flask experiment, with temperature (30 and 37 degrees C), pH (with or without CaCO3 tablets), and aeration (with or without baffles) as variable conditions. Yields varied 10-fold depending on the conditions investigated. |
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