Temperature and dissolved oxygen concentration as parameters of Azotobacter chroococcum cultivation for use in biofertilizers |
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Authors: | Božidar Šantek Vladimir Marić |
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Affiliation: | (1) Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6 / IV, 41000 Zagreb, Croatia |
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Abstract: | Summary Azotobacter chroococcum was grown in continuous culture at two temperatures (30 °C and 20 °C) and different dissolved oxygen tensions (DOT) (30 % to 40 % and 70 % to 80 % of air saturation), respectively. At the temperature of 30 °C and low DOT a relatively high volumetric productivity and efficiency of nitrogen fixation were obtained. After lowering the temperature to 20 °C, an intensive formation of cysts was observed associated with a drastic decrease of the bacterial growth. Bacteria in the form of cysts kept their physiological activity for a long period of time depending on temperature and preparation. |
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