The use of an amino acid analyzer for the rapid identification and quantitative determination of chitosan oligosaccharides. |
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Authors: | J J Chang J H Hash |
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Institution: | Department of Microbiology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 USA |
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Abstract: | The oligosaccharides of chitosan, glucosamine through chitohexaose, were separated and quantitated with the use of an amino acid analyzer. The oligosaccharides can be satisfactorilly resolved with a step gradient using two buffers, but resolution is improved with a linear buffer gradient. Using experimentally determined color factors, the method permits the rapid quantitation and identification of small amounts of chitosan oligosaccharides. |
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