Subgingival bacterial colonization profiles correlate with gingival tissue gene expression |
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| Authors: | Panos N Papapanou Jan H Behle Moritz Kebschull Romanita Celenti Dana L Wolf Martin Handfield Paul Pavlidis and Ryan T Demmer |
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| Institution: | (1) Division of Periodontics, Section of Oral and Diagnostic Sciences, College of Dental Medicine, Columbia University, New York, NY, USA;(2) Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY, USA;(3) Center for Molecular Microbiology and Department of Oral Biology, University of Florida, College of Dentistry, Gainsville, FL, USA;(4) Department of Psychiatry and Center of High-Throughput Biology, Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada |
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| Abstract: | Background Periodontitis is a chronic inflammatory disease caused by the microbiota of the periodontal pocket. We investigated the association
between subgingival bacterial profiles and gene expression patterns in gingival tissues of patients with periodontitis. A
total of 120 patients undergoing periodontal surgery contributed with a minimum of two interproximal gingival papillae (range
2-4) from a maxillary posterior region. Prior to tissue harvesting, subgingival plaque samples were collected from the mesial
and distal aspects of each tissue sample. Gingival tissue RNA was extracted, reverse-transcribed, labeled, and hybridized
with whole-genome microarrays (310 in total). Plaque samples were analyzed using checkerboard DNA-DNA hybridizations with
respect to 11 bacterial species. Random effects linear regression models considered bacterial levels as exposure and expression
profiles as outcome variables. Gene Ontology analyses summarized the expression patterns into biologically relevant categories. |
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