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Amino acid precursors for the detection of transketolase activity in Escherichia coli auxotrophs |
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| Authors: | Grégory Simon Madeleine Bouzon Franck Charmantray Virgil Hélaine Bertrand Légeret Philippe Marlière Laurence Hecquet |
| Affiliation: | 1. Clermont Université, Université Blaise Pascal, Laboratoire SEESIB, UMR 6504 CNRS, 63177 Aubière Cedex, France;2. CEA, DSV, IG, Genoscope, Laboratoire des Applications, 2 rue Gaston Crémieux, CP5706, Evry, F-91057, France;3. ISTHMUS, 31 rue de St-Amand, F-75015 Paris, France |
| Abstract: | Probes were developed for the in vivo detection of transketolase activity by the use of a complementation assay in Escherichia coli auxotrophs They combine the d-threo ketose moiety recognised by transketolase and the side chain of leucine or methionine. These compounds were donor substrates of yeast transketolase leading to the release of the corresponding α-hydroxyaldehydes which could be converted in E. coli by a cascade of reactions into leucine or methionine required for cellular growth. |
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